2004•Amino Acids & Biotic ResourcesRequires access

Determination of Aromatic Amino Acids by HPLC with UV Detection at Changeable Wavelength

Gong Aijun

Abstract

A method for determination of aromatic amino acids by RP-HPLC with UV detection at changeable wavelength was developed . Chromatographic conditions were as follows: mobile phase of phosphate buffer (pH6.0) and methanol in a ratio of 70: 30; flow rate of 0.6 ml/min. Samples were finally analyzed in 10min. Linear range of tyrosine, phenylalanine and tryptophane was 50-500μg·mL -1 , 200-20000μg·mL -1 and 20-2000μg·mL -1 . Their detection limit was 10μg·mL -1 、150μg·mL -1 and 5μg·mL -1 .

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Key concepts: Chromatography, Phenylalanine, High-performance liquid chromatography, Detection limit, Chemistry, Linear range, Aromatic amino acids, Amino acid