Protective Effect of Chrysalis Oil against Three Different Types of Liver Injury in Mice

Objective: To investigate the protective effect of chrysalis oil against three different types of liver injury in mice.Methods: A total of 150 male ICR mice were equally randomized into 3 groups by weight: group a(acute alcoholic hepatic injury group),group b(immunological liver injury group),and group c(D-galactosamine-induced liver injury group).Then the mice in each group were further randomly assigned into 5 subgroups: normal control,evening promise oil,liver injury control,low and high-dose chrysalis oil subgroups.After administration for 7 days,the mice in each group(except mice in the normal control subgroup) were treated as follows: the mice in group a were given 50% ethanol(12 mL/kg) to establish a mouse model of alcoholic liver injury;the mice in group b were intravenously injected with Bacille de Calmette Guerin(BCG) + lipidpolysaccharide(LPS) to induce a mouse model of immune liver injury;the mice in group c were intraperitoneally injected with D-GAL(600 mg/kg) to induce a mouse model of acute liver injury.Serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activities and liver malondialdehyde(MDA) and glutathione(GSH) content were measured 24 hours later.Results: The liver and spleen indexes,serum ALT and AST activities,and MDA and GSH content in the liver of mice from the chrysalis oil groups were significantly different to those of the liver injury control group(P 0.05).The liver and spleen indexes were significantly correlated with ALT and AST activities in the blood and MDA and GSH levels in the liver(P 0.05).Conclusion: Chrysalis oil has a significant protective effect against ethanol induced acute liver injury,BCG and LPS induced liver injury and D-GAL induced liver injury in mice.Its protective effect against alcoholic liver injury and BCG+LPS induced liver injury is greater than that of evening primrose oil.The mechanism may be related to its property to reduce lipid peroxidation in the body and its ability to prevent liver cells from necrosis.