2005•Chinese Journal of PharmaceuticalsRequires access

Determination of Deoxyribonucleic Acid by Resonance Light Scattering Method with Xylenol Orange

Li Liu

Abstract

A resonance light scattering(RLS)method for determination of deoxyribonucleic acid (DNA) was esta- blished by using xylenol orange as fluorescent probe. The RLS of DNA was greatly enhanced in pH range of 3.5 - 4.3 at wavelength of 374nm, and was proportional to the concentration of DNA in the range of 2.5 - 15μg/ml. The detection limit was 10ng/ml.

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Available abstract

Key concepts: Xylenol orange, Chemistry, DNA, Orange (colour), Detection limit, Fluorescence, Resonance (particle physics), Scattering