Effect of arsenic trioxide on the TRAIL roles of inhibiting the proliferation and regulating the expression of death receptor 4 and 5 gene in human lung cancer cell lines A549

Objective To investigate the effect of arsenic trioxide on the inhibiting proliferation against A549 cells roles of TRAIL and the possible mechanism.Methods The A549 cells were cultured to logarithmic phase in vitro and divided into As2O3 group,TRAIL group,combined group and the control group,then the As2O3 group were treated with As2O3 of 1,10 μmol/L,the TRAIL group was treated with TRAIL of 100 μg/L,the combined group was treated with 1 μmol/L As2O3 + 100 μg/L TRAIL and 10 μmol/L As2O3+100 μg/L TRAIL respectively,the control group was given DMEM.The 4 groups were continuely cultured for 24,48,72 hs,MTT method was used to detect inhibition ratio(IR) to A549 proliferation.DR4 and DR5 mRNA level of A549 cells were detected by RT-PCR.Results Compared with the As2O3 group and TRAIL group,the IR in the combined group increased significantly,especially higher after the treatment of 48 h and 72 h(all P0.05);the expression of DR4 and DR5 mRNA in combined group were obviously higher than those in As2O3 group and TRAIL group(all P0.05).Conclusions As2O3 can enhence TRAIL inhibits of human lung cancer cell line A549 growth by inducing death receptor 4 and 5 gene levels in vitro;the result provided a reasonable basis for targeting therapy of lung cancer.