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Effect of combination of arsenic trioxide and interferon on the expression of TRAIL and its receptor in U266 cells

Lingxiang Liu

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Abstract

Objective To investigate the feasibility of combination of arsenic trioxide(As2O3)and interferon α-2a in the treatment of multiple myeloma(MM).Methods U266 cells were randomly divided into four groups,three of which were treated with IFNα-2a and(or)As2O3 for 48 hours.The rate of apoptosis was assessed by Annexin-V-FITC and PI staining.The expression of TRAIL and its receptors DR4,DR5 were detected by flow cytometry.Results The rate of apoptosis,the expression of TRAIL and its receptor in As2O3 +IFNα-2a group were higher than those in the other three groups(P0.05).The expression of TRAIL and its receptor in the control group was lower than that in the other three groups(P0.05).Conclusion Both As2O3 and IFNα-2a can up-regulate the expression of TRAIL and its receptor,induce U266 cell apoptosis.This study provides new way for the treatment of MM.

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What this paper is about

Objective To investigate the feasibility of combination of arsenic trioxide(As2O3)and interferon α-2a in the treatment of multiple myeloma(MM).Methods U266 cells were randomly divided into four groups,three of which were treated with IFNα-2a and(or)As2O3 for 48 hours.The rate of apoptosis was assessed by Annexin-V-FITC and PI staining.The expression of TRAIL and its receptors DR4,DR5 were detected by flow cytometry.Results The rate of apoptosis,the expression of TRAIL and its receptor in As2O3 +IFNα-2a group were higher than those in the other three groups(P0.05).The expression of TRAIL and its receptor in the control group was lower than that in the other three groups(P0.05).Conclusion Both As2O3 and IFNα-2a can up-regulate the expression of TRAIL and its receptor,induce U266 cell apoptosis.This study provides new way for the treatment of MM.

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Available abstract

Objective To investigate the feasibility of combination of arsenic trioxide(As2O3)and interferon α-2a in the treatment of multiple myeloma(MM).Methods U266 cells were randomly divided into four groups,three of which were treated with IFNα-2a and(or)As2O3 for 48 hours.The rate of apoptosis was assessed by Annexin-V-FITC and PI staining.The expression of TRAIL and its receptors DR4,DR5 were detected by flow cytometry.Results The rate of apoptosis,the expression of TRAIL and its receptor in As2O3 +IFNα-2a group were higher than those in the other three groups(P0.05).The expression of TRAIL and its receptor in the control group was lower than that in the other three groups(P0.05).Conclusion Both As2O3 and IFNα-2a can up-regulate the expression of TRAIL and its receptor,induce U266 cell apoptosis.This study provides new way for the treatment of MM.

Key concepts: Arsenic trioxide, Apoptosis, Flow cytometry, Annexin, Receptor, Receptor expression, Interferon, Trioxide

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Effect of combination of arsenic trioxide and interferon on the expression of TRAIL and its receptor in U266 cells — Research Paper | ScholarLens