Research on the Impact of TRAIL on Apoptosis of SKOV3 Cell Line Ovarian Tumor Xenografts in Nude Mice

Objective To investigate the effects of Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) on the expression of Cysteine/aspartic acid specific protease-3(Caspase-3) in SKOV3 ovarian tumor cells and its relationship with the apoptosis of the ovarian tumor xenografts in nude mice.Methods Twenty-four nude mice with SKOV3 cell line ovarian tumor were randomly divided into four groups with 6 in each group.TRAIL(10 μg/kg) was given to the mice in the TRAIL group;DDP(3 μg/kg) was given to the mice in the DDP group;TRAIL(10 μg/kg) and DDP(3 μg/kg) were given to the mice in the TRAIL+DDP group;and 0.5 mL of saline solution was give to the mice in the control group.The expression of Caspase-3 was detected with immunohistochemistry.The apoptosis index(AI) of cells was determined by Terminal deoxynucleotidyl transferase mediated-dUTP nick end labeling(TUNEL).Results The expression of Caspase-3 in the TRAIL group(171.67±14.38),DDP group(172.50±14.75),and TRAIL+DDP group(230.00±40.99) was significantly higher than that in the control group(135.83±16.25)(P0.05).The apoptosis index for the control group,TRAIL group,DDP group and TRAIL+DDP group was 16.67±5.43,33.17±8.42,24.33±4.59,and 40.50±6.16,respectively.The apoptosis index for the TRAIL group and the TRAIL+DDP group was significantly higher than that in the control group and the DDP group(P0.05).Conclusion Soluble TRAIL has an effect on enhancing the expression of Caspase-3 in implanted tumor in nude mice.TRAIL protein can inhibit the growth of SKOV3 cells in nude mice by inducing cell apoptosis.