2012•Zhongguo shiyan fangjixue zazhiRequires access
Protective Effect of Quercetin on Endothelial Cell Injured by H_2O_2
Wenna Chen
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Abstract
Objective:To evaluate the effects of quercetin on the injured vascular endothelial cells and its mechanism.Method:Endothelial cell culture was in vitro.The endothelial cell injury model was established by cultured with H2O2 for 18 h,Quercetin at dose of 8,4,2 mg·L-1 was cultured with the cells before the model establishment.Flow cytometry was used for determining endothelial cell death rate.Endothelial cell was detected by ELISA for medium thrombomodulin(TM) content;spectrophotometric determination was used for the culture medium lactate dehydrogenase(LDH) activity.Result:Compared to the model group,quercetin can lower endothelial cell death rate,reduce the content of TM and activity of LDH in the culture medium.Conclusion: Quercetin can obviously protect endothelial cells,and its protective effect is related to against lipid peroxidation,protection of the cell membrane.