Purification and Stability of Polyphenols from Ageing Lotus Seed Epicarp

In this study,the static adsorption and desorption properties of six macroporous resins were compared for the purification of lotus seed epicarp polyphenols( LSEPPs). The purification conditions of the most suitable macroporous resin were optimized. In addition,the stabilities of LSEPPs were also investigated. The results indicated that AB-8 resin had the optimal adsorption and desorption performance. The optimal adsorption conditions with AB-8 macroporous resin were determined as follows: solution concentration of 4. 5 mg / mL,feeding rate of 0. 5 mL / min,and the optimal desorption conditions with AB-8 macroporous resin were ethanol concentration of 50%,elution rate of 2 mL / min,elution volume of 4 BV. Under these conditions,the purity of LSEPPs was 69. 34%. The stabilities of LSEPPs were improved by low temperature,light-avoiding and midly acidic conditions. Fe3 +,Fe2 +,Al3 +,Sn2 +,Cu2 +and Pb2 +had destructive effect on LSEPPs.