Study on Isolation and Enrichment of Barley Polyphenols by Macroporous Resin

Static adsorption capacity and static desorption rate were assayed to screen the proper macroporous resin for barley polyphenols, and most suitable manipulation parameters of macroporous resin were confirmed. The results showed that AB-8 macroporous resin has strong adsorption capacity and easy desorption property among the six resins used in the experiment. The most appropriate adsorption conditions are: sample concentration 1.0 mg/ml, pH value 6.0 and sample flow velocity 2～ 3 BV/h. The most fitting elution conditions are: 70% ethanol concentration, elution rate 2 BV/h and elution volume 3.0 BV. After the isolation and enrichment of AB-8, the contents of polyphenols increase 3 times and the reducing power and DPPH radical inhibition are improved obviously.