Effects of "Qi-Enriching and Blood-Stasis-Resolving Decoction" on Ultrastructure of Chondrocyte in vitro of Cervical Intervertebral Discs in Rats
Lizhong Xu
Abstract
Lizhong Xu
Abstract
To study the effects of Qi-Enriching and Blood-Stasis-Resolving on ultrastructure of chondrocyte,70 SD rats were evenly randomized into five groups and then treated accordingly.Four days later,the serum in each group was collected and added it to the culture in vitro of chondrocyte;the ultrastructure and apoptosis rate of the chondrocyte were observed by transmission electronic microscope and flow cytometer.Results showed that this Decoction could promote the DNA synthesis of chondrocyte,with significant differences compared with other control groups( P 0.01).It is indicated that this Decoction can precipitate the growth,proliferation and DNA synthesis of chondrocyte.
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To study the effects of Qi-Enriching and Blood-Stasis-Resolving on ultrastructure of chondrocyte,70 SD rats were evenly randomized into five groups and then treated accordingly.Four days later,the serum in each group was collected and added it to the culture in vitro of chondrocyte;the ultrastructure and apoptosis rate of the chondrocyte were observed by transmission electronic microscope and flow cytometer.Results showed that this Decoction could promote the DNA synthesis of chondrocyte,with significant differences compared with other control groups( P 0.01).It is indicated that this Decoction can precipitate the growth,proliferation and DNA synthesis of chondrocyte.
Key concepts: Chondrocyte, Ultrastructure, Decoction, In vitro, Medicine, Andrology, Anatomy, Pathology