Investigation on the Measurement of Insoluble Yeast Glucan Content

The measurement methods of insoluble yeast glucan content were investigated.The purity of glucan was usually expressed by monosaccharide content after acid hydrolysis.Acid concentration in hydrolysis,the temperature and the time were important factors influencing the measurement results of monosaccharide content.The hydrolysis by H2SO4 and TFA under different hydrolysis conditions was compared to analyze their influence on the measurement of insoluble yeast glucan content.The results showed that H2SO4 was preferable than TFA as hydrolysis acid and the best hydrolysis conditions were as follows:2.7 mol/L H2SO4 concentration,hydrolysis temperature at 100 ℃,and 3 h hydrolysis time.