Optimization of Ultrasonic-assisted Extraction of Polyphenol from Litsea Coreana using Response Surface Methodology

Based on single factor test,a 3-factor,3-level Box-Behnken experimental was adopted,including dependent variables( extraction time,liquid-solid ratio and ultrasonic wave power) and independent variable( polyphenol yield).The optimum conditions of ultrasonic-assisted extraction technology for polyphenol from Litsea coreana were determined by using response surface method.The results showed that the regression model fitted well with experimental data.The optimum conditions were 5 g samples,extraction time 22 min,liquid-solid ratio 21:1,ultrasonic wave power 300 W.Under these conditions,the polyphenol yield was 3.76%.