Optimization of technology for ultrahigh pressure extraction of total flavonoids from Litsea coreana Level.

Ultrahigh pressure extraction(UHPE) of total flavonoids from Litsea coreana Level.was investigated.The effects of different pressure,pressure holding time,ethanol concentration and liquid-solid ratio on the extraction yield of total flavonoids were optimized with Response Surface Methodology(RSM).A mathematical model was established and analyzed to describe the relationships between the studied factors and the response of the extraction yield of total flavonoids.The results indicated that the optimum extraction parameters are as follows:extraction pressure 427 MPa,pressure holding time 9min,liquid-solid ratio 41 mL/g,ethanol concentration 70 percent.Under such conditions,the experiment value of yield was(6.263±0.014)%(n=3),which was close to the estimated value(6.281%) attained by using regression model.Compared to the traditional heating method,the ultra high pressure extraction process was a suitable method for its higher yield and shorter extraction time.