2003•Research and Practice on Chinese MedicinesRequires access

Determination of Hesperidin in Xiaoshizhixie Power by HPLC

Zhicheng Cai

Abstract

Objective To establish the method for determining hesperidin in Xiaoshizhixie Power by HPLC.Methods Samples were extracted by ultrasonic wave;HPLC was used to determine the content of hesperidin in samples on Kromasil C 18 column with acetonitrile water phosphoric acid(180∶820∶0.1) as the mobile phase.The detection wavelength was 283 nm.The column temperature was 28 ℃.Results The linear range of hesperidin was 0.197 μg～1.182 μg(r=0.999 9,n=6).The average recovery was 99.8%,RSD was 1.3%.Conclusion The method is accurate,simple and quick.It can be used for quality control of Xiaoshizhixie Power.

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Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Phosphoric acid, Chemistry, Linear range, Acetonitrile, Detection limit