Study on Culture Conditions of Cordyceps militaris Fermentation Mycelium

[Objective]To provide reference for the production of effective medicinal ingredient by Cordyceps militaris in liquid fermentation. [Method]Through single factor test,the best culture conditions of Cordyceps militaris was determined by changing carbon source,nitrogen source,age of pure culture,initial pH of medium,culture time,temperature,stirring rate and filling liquid. [Result]The optimal culture conditions: the optimal carbon source was 2% sucrose and the optimal nitrogen source was 1% tryptone. The best fermentation conditions of flask were: age of pure culture 36h,initial pH 6. 5,fermentation 6 d,temperature 24℃,stirring rate 200 r / min,filling liquid 150 ml /250 ml. [Conclusion]The best culture conditions for Cordyceps militaris C- 7 mycelium were obtained,which will provide basis for further development and utilization.