Submerged Fermentation of Cordyceps militaris

In this experiment,the best formula of carbon,nitrogen nutrition source and culture conditions on liquid medium of Cordyceps militaris were optimized using cordyceps militaris mycelia biomass as target by single factor contrast experiment and orthogonal test.The optimal liquid medium of Cordyceps militaris were selected,sucrose was the best carbon source,followed by maltose,Yeast extract was the best nitrogen source,followed by peptone.The suitable formula for liquid medium of Cordyceps militaris were confirmed,sucrose 3%,yeast extract 3%,KH2PO4 0.1%,MgSO4 0.15%;The optimum temperature was 24 ℃,pH 5.5,inoculation was 15%,rotate of the flask at 140 r/m,ventilation of the fermentor at 1∶1.2～1∶1.6 V/(V·min).This study provides a theoretical basis for liquid culture of the Cordyceps militaris.