Purification of the total flavonoids from Chrysanthemum indicum with DM130 macroporous resin

Objective To investigate the conditions of purifying total flavonoids from Chrysanthemum indicum with macroporous adsorption resin.Methods Static and dynamic tests of macroporous resin were employed to study on the purification effect and influencing factors with the recovery rate of total flavonoids as index.Results The purification conditions of DM130 resin were as follows:the concentration of total flavonoids was 2.78 mg/mL at pH3-4,80% alcohol eluting for 5 times as much as the resin volume,and the elution velocity was 2 mL/min.The recovery of total flavonoids was 79.19%,and the purification rate was 37.31%.Conclusion The optimum technology is stable and feasible,and DM130 resin is suitable for separating and purifying total flavonoids from Chrysanthemum indicum.