Separation and Purification of Flavonoids from Chrysanthemum Indicun with Macroporous Resin

Objective:A method for separation and purification of flavonoids from chrysanthemum indicun with macroporous resin was studied.Method:By using chrysanthemum indicun in Guizhou and with the content and recovery rate of flavonoids as indexes and the static adsorption capacity and the static elution ratio.Result:The results are as flollows:The static adsorption capacity of AB-8 type resin was 114.65mg-1g,the static elution ratio were 94.9%,the dynamic adsorption capacity of AB-8 type resin was 94.5mg-1g,the recovery rate is more than 92.6% and the purity is more than 90%.AB-8 type resin is the best for separating and purificating chrysanthemum indicun in flavonoids.Conclusion:The optimum conditions is:AB-8 type macroporous resin,the eluant is 70% alcohol and 2～3 times as the volume of the resin,the volume of the resin is 10.6 times of flavonoids in sample,concentration of flavonoids of sample is 19.8mg-1ml and current velocity of 2～3ml-1min,pH value of sample is 4～5.