2006•Di-Si Junyi Daxue xuebaoRequires access
Cloning and sequencing of recombinant plasmid affecting survivin gene translation by RNA interference technique
Cong Shi
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Abstract
AIM: To clone the recombinant plasmid affecting survivin gene translation by RNA interference and to analyze the nucleic acid sequence of the recombinant plasmid for new gene therapy for tumors. METHODS: Two DNA sequences containing small hairpin structure were designed and synthesized. The complement form was obtained by annealing and being cloned into vector pSilencer 3.1-H_1- hygro and the recombinant plasmid was transformed into strain DH5α. The plasmid identified by restriction enzyme was used for sequencing. RESULTS: The recombinant plasmid was cloned and the aimed sequence was obtained. CONCLUSION: Successful cloning of the recombinant plasmid helps to find a new gene therapy for tumors.