Constructing and sequence analyzing of transcriptional small hairpin RNA recombinant plasmid targeting bcl-2 gene

Objective To construct the recombinant plasmids expressing connective gene bcl-2 short hair-pin RNA(shRNA) by Pgenesil-1 plasmid vector for the further searching new gene therapy method of the tumors.Methods Two DNA sequences containing small hairpin structure were designed and synthesized.Annealing and inserted into Pgenesil-1.The recombinant plasmid was transformed into DH5a strain.Then the recombinant plasmid identified by restriction enzyme was used for sequence analysis.Results The recombinant plasmid targetintg bcl-2 gene was constructed and the aim sequence was obtained.Conclusion Successfully constructing the recombinant plasmid make it possible searching new gene therapy method of tumors.