Cloning and sequence analyzing of the recombinant plasmid affecting gene MBD2 translation by RNA interfering

Objective For the further searching new gene therapy method of the tumors,the recombinant plasmid affecting gene MBD2 translation by RNA interfering was cloned and the nucleic acid sequence was analysed.Methods Two DNA sequences containing small hairpin structure were designed and synthesized.The complement form was obtained by annealing and inserted into vector pTZU6+1.The recombinant plasmid was transformed into Jm109 strains.Finally the plasmid identified by restriction enzyme was used for sequence analysis.Results The recombinant was cloned and the aim sequence was obtained.Conclusion Successfully cloning the recombinant make it possible searching new gene therapy method of the tumors.