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STUDY ON THE EXTRACTION OF RICE PROTEIN BY ENZYMES

Li Zeng

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Abstract

The rice protein was extracted by 4 kinds of protease enzymes.Alcalase was the optimal hydrolysis enzyme with the compare of extraction efficiency.The effects of reaction temperature,solid-liquid ratio,reaction time,added enzyme amount and pH on extraction efficiency of the hydrolysis of rice protein were considered in the extracting process.Then through the orthogonal experiment,the optimum technological conditions were as follow:temperature 60 ℃,pH 9.5,enzyme amount(E/S)1.5 %,solid-liquid ratio l∶6 and time 4 h.In these conditions,the extraction efficiency of protein was 76.42 %.

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What this paper is about

The rice protein was extracted by 4 kinds of protease enzymes.Alcalase was the optimal hydrolysis enzyme with the compare of extraction efficiency.The effects of reaction temperature,solid-liquid ratio,reaction time,added enzyme amount and pH on extraction efficiency of the hydrolysis of rice protein were considered in the extracting process.Then through the orthogonal experiment,the optimum technological conditions were as follow:temperature 60 ℃,pH 9.5,enzyme amount(E/S)1.5 %,solid-liquid ratio l∶6 and time 4 h.In these conditions,the extraction efficiency of protein was 76.42 %.

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Available abstract

The rice protein was extracted by 4 kinds of protease enzymes.Alcalase was the optimal hydrolysis enzyme with the compare of extraction efficiency.The effects of reaction temperature,solid-liquid ratio,reaction time,added enzyme amount and pH on extraction efficiency of the hydrolysis of rice protein were considered in the extracting process.Then through the orthogonal experiment,the optimum technological conditions were as follow:temperature 60 ℃,pH 9.5,enzyme amount(E/S)1.5 %,solid-liquid ratio l∶6 and time 4 h.In these conditions,the extraction efficiency of protein was 76.42 %.

Key concepts: Extraction (chemistry), Enzyme, Hydrolysis, Protease, Chemistry, Chromatography, Neutral protease, Protein purification

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