Significance of expression of NF-κB,ICAM-1,MMP-2 and MMP-9 in renal hypertension rats with focal cerebral ischemia-reperfusion injury

Objective To observe the Expression of NF-κB,ICAM-1,MMP-2 and MMP-9 in renal hypertension rats with focal cerebral ischemia-reperfusion injury,and to research the mechanism of hypertension increased ischemic brain injury.Methods 20 male Wistar rats were randomly divided into two groups with hypertension and normal blood pressure group.The former group was treated with renal artery stenosis methods to perform rat model of renal hypertension.Normal blood pressure group was further divided into sham-operation group(N)and ischemia-reperfusion group(IR),received sham surgery and ischemia-reperfusion treatment,respectively.Using the intraluminal suture method of middle cerebral artery to induce cerebral ischemia-reperfusion model of ischemia time was 2h and reperfusion 22h.The expression of NF-κBp65,ICAM-1,MMP-2 and MMP-9 in the brain were detected by immunohistochemistry,and the gray value was measured by image analysis system.Results In the N group,IR group of NF-κBp65,ICAM-1,MMP-2 and MMP-9 showed significantly higher gray value(P0.01);In the IR group,HIR group of NF-κBp65,ICAM-1,MMP-2 and MMP-9 gray value increased(P0.05).Conclusion Hypertension aggravates ischemia-reperfusion brain tissue NF-κBp65,ICAM-1,MMP-2 and MMP-9 expression.