Effects of cerebral ischemic reperfusion on the expression of NF-κBp65 and MMP-9 in diabetic rats

Objective To investigate the expression of cerebral nuclear transcription factor (NF)-KBp65, matrix metalloproteinase-9 (MMP-9), and their clinical significance in diabetic rats with cerebral is-chemic reperfusion. Methods Eighty-nine healthy Wistar rats were randomly divided into four groups: normal control group, sham operated group, normoglycemic cerebral ischemic reperfusion group (nIR) and diabetic cerebral ischemic reperfusion group (dIR), reperfusion for 0 h, 6 h, 12 h and 24 h. Diabetic model was made by injection of streptozotocin (STZ) through abdomen. The model of cerebral ischemia reperfusion was developed by thread embolism method. The expression of NF-KBp65 and MMP-9 were detected by immuno-histochemistry. Results The expression of NF-KBp65 and MMP-9 in both dIR group and nIR group after reperfusion was mainly around ischemic district. The percentage of positive cell indicated by NF-κBp65 increased in dIR group, which were (24.68±4.19)%, (53.37±7.86)%, (72.38±7.24)%, (60.66±5.02)% in reperfusion for 0h, 6h, 12h, 24h respectively, compared with the same time in nIR group (P0.01). The number of positive blood capillary indicated by MMP-9 increased in dIR group, which were (0.83±0.81), (2.44±1.61), (5.08±2.11), (3.63±1.60) in reperfusion for 0h, 6h, 12h, 24 h respectively, compared with the same time in nIR group (P0.01), except the subset Oh and 24 h (P0.05), and the expression of MMP-9 positive in subset 24 h was lower than that in subset 12 h (P0.05). There was a correlation of time with the expression of NF-κBp65 and MMP-9 around cerebral ischemia district. Conclusion In diabetic cerebral ischemic reperfusion rats the expression of NF-κBp65 and MMP-9 increased and advanced, which may be a mechanism of the cerebral ischemic reperfusion injury aggravated by diabetes.