Effects of enalapril on expression of MMP-2 and MMP-9 in renal hypertension rats with focal cerebral ischemic reperfusion

Objective To observe the effects of ACEI（ enalapril） on expression of MMP-2 and MMP-9 in renal hypertension rats with focal cerebral ischemia-reperfusion injury. Methods Twenty-eight Wistar male rats were randomly divided into two groups： hypertension group and normaltension group. The former which formed model of renal hypertension rats by constricted renovascular were randomly divided into enalapril group （Y） and hypertension ischemia and reperfusion group（HIR）,which fed with enalapril 2mg/kg and equal volum saline respectively;The latter were divided into sham-operation group （ N） and normaltension ischemia and reperfusion group （IR）. The focal cerebral ischemia model was established in Wistar rats by using the method of thread inserting left middle cerebral artery occlusion（MCAO） for 2h. After ischemic 24h,the expression of MMP-2 and MMP-9 were measured by immunohisto-chemistry,and the gray scale value was measured by imaging analysis. Results Compared with N,the gray scale values of MMP-2 and MMP-9 in IR were higher（P 〈0. 01）; Compared with IR, the gray scale values of MMP-2 and MMP-9 in HIR were higher（P〈0. 05）;Compared with HIR,the gray scale values of MMP-2 and MMP-9 in Y were lower（P〈0.01）. Conclusion Hypertension can increase the expression of MMP-2 and MMP-9 in rats with focal cerebral ischemia-reperfusion. ACEI （Enalapril） could inhibit the expression of MMP-2 and MMP-9 in renal hypertension rats with focal cerebral ischemia-reperfusion. Key words: Hypertension renal;  Reperfusion injury;  Enalapril;  Matrix metalloproteinase-2;  Matrix metallopro-teinase-9