Evaluation of transfection efficiency and cytotoxicity of self-prepared polyamine cationic liposome

AIM: To evaluate the transfection efficiency and cytotoxicity of polyamine cationic liposome(PCL)prepared by ourselves. METHODS: PCL with polyamine cationic lipids TC-Chol and neutral phospholipid DOPE at a molar ratio of 3∶1 was prepared,and PCL or Lipofectamine 2000 (as control) mediated by the plasmid PIRES2-EGFP was transfected into HeLa cells or Hep2 cells,and the expression of EGFP was determined by fluorescence microscope.The survival fraction was determined by MTT to evaluate the transfection efficiency and cytotoxicity after transfection. RESULTS: The transfection efficiency of PCL prepared with TC-Chol and DOPE at a molar ratio of 3∶1 was a few lower than that of Lipofectamine 2000,but the cytotoxicity of the former was less than the latter. CONCLUSION: PCL has a sound transfection activity and less cytotoxicity compared with Lipofectamine 2000.It is shown to be promising for gene transfection and gene therapy.