Effects of Cell Types on Gene Transfection Mediated by Cationic Liposome

[Objective] The research aimed to provide references for the research on non-viral vector cationic lipids.[Method] With cationic liposome Lipofectamine 2000 as vector,the transfection efficiency of Lipofectamine 2000 to different cells(HeLa cell,B16 cell,MCF-7 cell and SW-480 cell) were studied through DNA delay test and plasmid transfection test and it was compared with that of other transfection reagents(Sofast and DOTAP).And the effects of cell types on the transfection efficiency of Lipofectamine 2000 were discussed.[Result] With the increasing of the proportion of transfection reagent in the complex,DNA delay action was strengthened.When the mass ratio of Lipofectamine 2000 to DNA was increased to 5:1,only a few DNA moved out from the origin site.The transfection efficiency of Lipofectamine 2000 to SW-480 cell was obviously higher than other 3 kinds of cell types.The transfection effeiciency of Lipofectamine 2000 on B16 cell line was better than that of Sofast and that of Sofast and DOTAP was equivalent.[Conclusion] Lipofectamine 2000 had high transfection efficiency to most cells,but it was not suitable for all types of cells.