Construction of polyamine cationic liposome transfection system

[Objective] To prepare polyamine cationic liposome(PCL), and transfect it into mammlian cell to assess transfection efficiency and cytotoxicity, and to optimize the condition of transfection. [Methods] PCL with polyamine cationic lipids TC-Chol and neutral phospholipid DOPE at a certain molar ratio was prepared, the plasmid PIRES2-EGFP which mediated PCL was transfected into Hela cells, and the expression of EGFP was determined by fluorescence microscope. The survival fraction rate was determined by MTT to evaluate the cytotoxicity post-transfection for optimization of the transfection condition of mammalian cell. [Results] PCL mediated transfection could enhance the transfection efficiency, at the mass ratio of 3～4∶1 and it was more efficient and less cytotoxic. [Conclusion] PCL prepared with TC-Chol and DOPE can enhance the transfection efficiency, and satisfactory transfection activity and less cytotoxicity is observed under certain condition. It provides laboratary data for gene transfection and gene therapy.