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2004•Journal of Apoplexy and Nervous DiseasesRequires access

The expression of phospho-cAMP response element binding protein in hippocampus of rats reperfusion following global ischemia

Yonghai Liu

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Abstract

Objective To explore the expression of pCREB in hippocampus of rats reperfusion following global ischemia. Methods Adult male Sprague-Dawley (SD)rats were subjected to the 15min transient cerebral ischemia induced by the four-vessel occlusion method. Using Western Blotting and immunohistochemistry with anti-phospho-CREB antibody,we studied that hippocampd neurons' injury,changes of pCREB content and its distribution. Results Immunohistochemistry staining manifested the number of pCREB positive cells in the I/R group reached the maximum at 3h and declined quickly to the Sham group level at 24h in the CA 1 subfield. While there were stronger and longer expressions in the DG subfield. Western Blot analysis showed pCREB expression level reached the peak at 3h in I/R group,and decreased in a time-dependent manner. Conclusion Global ischemia-reperfusion promotes the expression of pCREB,which maybe involved in protection after cerebral ischemia.

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Objective To explore the expression of pCREB in hippocampus of rats reperfusion following global ischemia. Methods Adult male Sprague-Dawley (SD)rats were subjected to the 15min transient cerebral ischemia induced by the four-vessel occlusion method. Using Western Blotting and immunohistochemistry with anti-phospho-CREB antibody,we studied that hippocampd neurons' injury,changes of pCREB content and its distribution. Results Immunohistochemistry staining manifested the number of pCREB positive cells in the I/R group reached the maximum at 3h and declined quickly to the Sham group level at 24h in the CA 1 subfield. While there were stronger and longer expressions in the DG subfield. Western Blot analysis showed pCREB expression level reached the peak at 3h in I/R group,and decreased in a time-dependent manner. Conclusion Global ischemia-reperfusion promotes the expression of pCREB,which maybe involved in protection after cerebral ischemia.

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Available abstract

Objective To explore the expression of pCREB in hippocampus of rats reperfusion following global ischemia. Methods Adult male Sprague-Dawley (SD)rats were subjected to the 15min transient cerebral ischemia induced by the four-vessel occlusion method. Using Western Blotting and immunohistochemistry with anti-phospho-CREB antibody,we studied that hippocampd neurons' injury,changes of pCREB content and its distribution. Results Immunohistochemistry staining manifested the number of pCREB positive cells in the I/R group reached the maximum at 3h and declined quickly to the Sham group level at 24h in the CA 1 subfield. While there were stronger and longer expressions in the DG subfield. Western Blot analysis showed pCREB expression level reached the peak at 3h in I/R group,and decreased in a time-dependent manner. Conclusion Global ischemia-reperfusion promotes the expression of pCREB,which maybe involved in protection after cerebral ischemia.

Key concepts: CREB, Ischemia, Western blot, Immunohistochemistry, Hippocampus, Blot, Internal medicine, Endocrinology

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