2009•Chinese Journal of Minimally Invasive NeurosurgeryRequires access
Expression of autophagy-associated protein Beclin 1 in the hippocampus after global brain ischemia-reperfusion injury in rats
Xiaoqiang Wang
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Abstract
Objective To study the expression of autophagy-associated protein Beclin 1 and its significance in the hippocampus after global cerebral ischemia-reperfusion injury in rats.Methods The global cerebral ischemia was induced by four-vessel occlusion in Sprague-Dawley(SD) rats.The rats were randomly divided into sham-operated group and ischemia-reperfusion group.Fifteen minutes after the cerebral ischemia,the reperfusion was performed at 0 and 30 min,3,6,12 and 24 h,and 1 and 3 d.The expression of Beclin 1 in the hippocampus after ischemia-reperfusion was detected by immunoblotting analysis at each time point.Results Compared with the sham-operated group,the expression level of Beclin 1 reached a peak at 1 d after cerebral ischemia-reperfusion(P0.05).Conclusion Global cerebral ischemia-reperfusion injury induces up-regulation of Beclin 1 in rat hippocampus,which demonstrates up-regulation of autophagic activity in rat hippocampus after cerebral ischemia and reperfusion.