2006•Jiepou kexue jinzhanRequires access
Upregulatory Effect of NGF on the Expression of CREB and phospho-CREB Protein During Focal Cerebral Ischemia/Reperfusion in Rat Hippocampus
Fang Xiu-bin
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Abstract
Objective To study the effect of nerve growth factor(NGF) on the expression of cyclic AMP response element binding protein(CREB) and phosphorylated-CREB in rat hippocampus with focal cerebral ischemia/reperfusion(I/R).Methods Focal cerebral ischemia/reperfusion model was induced by occlusion of the middle cerebral artery using the intraluminal suture method.The expression of CREB and phospho-CREB in the hippocampus CA1 region was detected with immunohistochemistry and Western bloting and analyzed by image analysis system.Results There was definite expression of CREB in CA1 region of hippocampus in sham group,while it was lesser in I/R group than in sham group and more in NGF group than in I/R group(P0.05).Phospho-CREB positive product was barely detected in CA1 region of hippocampus in sham group and became more in I/R group than in sham group.And the expression of phospho-CREB got more in CA1 region in NGF group than in I/R group(P0.05).Conclusion NGF upregulates the expression of CREB and phospho-CREB in the ischemic neurons of the hippocampus after focal cerebral ischemia/reperfusion and CREB involves in the mechanism of protective role of NGF to ischemic neurons.