Isolation,identification of a alkyl-polyoxyethylene degrading bacterium strain and its degradation characteristics.

A bacterial strain B30 of degrading 12 alkyl-polyoxyethylene (Brij-30) was isolated, purified and sorted from the wastewater in pollution-outlets of a factory, and identified as Pandoraea sp.. At temperature of 30℃, pH of 5~8, Brij-30 concentration lowered 0.20g/L, protein peptone as the nitrogen source, bacterial strain B30 degradation effect was the best. Zn2+、Ca2+、Al3+、Fe3+ had greater promoting effect on the growth of bacterial strain B30 and its degradation property. At low concentration area (0.05~0.20g/L), bacterial strain B30 grew the quickest, degradation activity was the strongest, could degrade basically Brij-30 in 12h; in medium concentration area (about 0.40g/L), could degradate 50% Brij-30 in 72h; in high concentration area (0.80~1.50g/L), the degradation rate in 72h was lower under 30%.