Isolation,Identification and Characteristics of PCB77-degrading Strain

A degrading bacteria strain was isolated from polluted soil by polychlorinated biphenyls(PCBs).The degrading bacteria strain's 16S rDNA was amplified with bacteria universal primer,the amplified product(about 1,500 bp)was purified and sequenced for phylogenetic analyses with Genbank.The bacterium was identified as Pseudomonas sp.which was used to the degradation of PCB77,The results of the study showed that the bacteria reached logarithmic phase on the second day,and the degradation ratio of PCB77 was 58.63% when the temperature was 30 ℃,pH was 7.0,microorganism inoculation amount was 109 cfu/mL and the concentration of PCB77 was 1.0 mg/L.The optimum reaction conditions for PCB77 degradation were pH 7.0 of the solution,external carbon source 2 g/L,microbial inoculation amount 2×109 cfu/mL and the initial concentration of PCB77 0.5 mg/L.