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The Determination of Yolk Phospholipids by Reversed Phase -High Performance Liquid Chromatography

Baoquan Liu

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Abstract

A successful method for the determination of yolk phospholipids by reversed phase -high performance liquid chromatography is developed. The samples were separated by RP-HPLC on column HYPERSIL C18 5靘 (250mm?.6mm ID). The best condition for determination is as follows: mobile phase is acetonitrile , methanol , water (40:40:20 v/v) ; flow rate is 1.2mL/min ; column temperature is 30℃; detector is SPD-M10A (SHIMADZU). The absorption of the chloroform (the solvent) and all the samples were determined in the ranges of 200nm to 250nm; and UV 205nm is best.

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What this paper is about

A successful method for the determination of yolk phospholipids by reversed phase -high performance liquid chromatography is developed. The samples were separated by RP-HPLC on column HYPERSIL C18 5靘 (250mm?.6mm ID). The best condition for determination is as follows: mobile phase is acetonitrile , methanol , water (40:40:20 v/v) ; flow rate is 1.2mL/min ; column temperature is 30℃; detector is SPD-M10A (SHIMADZU). The absorption of the chloroform (the solvent) and all the samples were determined in the ranges of 200nm to 250nm; and UV 205nm is best.

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Available abstract

A successful method for the determination of yolk phospholipids by reversed phase -high performance liquid chromatography is developed. The samples were separated by RP-HPLC on column HYPERSIL C18 5靘 (250mm?.6mm ID). The best condition for determination is as follows: mobile phase is acetonitrile , methanol , water (40:40:20 v/v) ; flow rate is 1.2mL/min ; column temperature is 30℃; detector is SPD-M10A (SHIMADZU). The absorption of the chloroform (the solvent) and all the samples were determined in the ranges of 200nm to 250nm; and UV 205nm is best.

Key concepts: Chromatography, High-performance liquid chromatography, Acetonitrile, Solvent, Methanol, Chemistry, Chloroform, Phase (matter)

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