Separation and quantitative determination of monoacylglycerol mixtures by reversed phase HPLC

Abstract The separation and quantitative determination of underivatized saturated and unsaturated monoacylglycerol were examined by reversed phase high performance liquid chromatography using acetonitrile/water as the mobile phase. Monoacylglycerol (MG) mixtures containing saturated MG (C6‐MG‐C18‐MG), monooleoylglycerol and monolinoleoylglycerol were sufficiently resolved, but mixtures of 1‐MG and 2‐MG with the same acyl group could not be separated under the conditions given below: column, C‐8 chemical bonded column (4.6 × 250 mm); sample solvent, diethyl ether (UV detector at 210–215 nm) or chloroform; mobile phase, acetonitrile/water (7:3, v/v). Quantitative determinations were made for mixtures of three components (representative standard MGs) on a reversed phase column (Unisil Q, C‐8) using a UV detector and the internal standard method. Standard deviation and coefficient of variation gave accurate, reproducible results.