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2006•Zhongguo nongye KexueRequires access

Studies on Genetic Diversity and Relationship in Tobacco Germplasms by AFLP Analysis

Zhu Lie-shu

Open publisher page 3 citations

Abstract

【Objective】This study was carried out in order to analyze genetic diversity and relationship in tobacco germplasms.【Method】Genomic DNA of 48 tobacco materials were amplified by 4 AFLP selective primers(EcoRI+3/MseI+3).【Result】 A total of 321 AFLP bands were obtained including 174 polymorphic ones.The percentage of polymorphic bands is 54.2%.UPGMA cluster analysis based on AFLP molecular markers data divided 48 varieties into two groups: N.tobacum L group and N.rustica L.group.N.tobacum L.group could be classified into four sub groups.The genetic distance of 48 cultivars was between 1.41 and 11.0.【Conclusion】The results showed that AFLP approach is efficient in generating more accurate information on genetic background,relationship and evolution of tobacco germplasms.

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【Objective】This study was carried out in order to analyze genetic diversity and relationship in tobacco germplasms.【Method】Genomic DNA of 48 tobacco materials were amplified by 4 AFLP selective primers(EcoRI+3/MseI+3).【Result】 A total of 321 AFLP bands were obtained including 174 polymorphic ones.The percentage of polymorphic bands is 54.2%.UPGMA cluster analysis based on AFLP molecular markers data divided 48 varieties into two groups: N.tobacum L group and N.rustica L.group.N.tobacum L.group could be classified into four sub groups.The genetic distance of 48 cultivars was between 1.41 and 11.0.【Conclusion】The results showed that AFLP approach is efficient in generating more accurate information on genetic background,relationship and evolution of tobacco germplasms.

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Available abstract

【Objective】This study was carried out in order to analyze genetic diversity and relationship in tobacco germplasms.【Method】Genomic DNA of 48 tobacco materials were amplified by 4 AFLP selective primers(EcoRI+3/MseI+3).【Result】 A total of 321 AFLP bands were obtained including 174 polymorphic ones.The percentage of polymorphic bands is 54.2%.UPGMA cluster analysis based on AFLP molecular markers data divided 48 varieties into two groups: N.tobacum L group and N.rustica L.group.N.tobacum L.group could be classified into four sub groups.The genetic distance of 48 cultivars was between 1.41 and 11.0.【Conclusion】The results showed that AFLP approach is efficient in generating more accurate information on genetic background,relationship and evolution of tobacco germplasms.

Key concepts: Amplified fragment length polymorphism, UPGMA, Genetic diversity, EcoRI, Biology, Genetics, Genetic relationship, Genetic variation

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