Study on Extraction of Flavonoids from the Bitter Melon Assisted by Surfactant

Objective To improve the extracting efficiency of total flavonoids from bitter melon,and filter out the best extracting process.Methods Experiments were carried out to explore the extraction of flavonoids from the bitter melon.The effects of surfactant usage,extracting temperature,extracting duration,ethanol volume percentage on extraction effect of total flavonoids from bitter melon were discussed.L9(34)orthogonal experiment was used to obtain the optimum extraction conditions of total flavonoids.Results Tween-20 was suit to explore the extraction of flavonoids from the bitter melon.Various factors on extraction effect sizes was as following:surfactant usageextraction temperature ethanol volume percentageextracting duration.Conclusion The optimum extraction conditions were obtained as ethanol concentration 70%,Tween-20 0.05g,extraction at 70℃for 4 hours.