MicroRNA-146a Inhibits the Proliferation of Glioma Cell

Objective: To investigate the abnormally expression and effect of miR-146a on proliferation in glioma cancer.Methods: Real-Time PCR assay was used to determine the abnormally expression of miR-146a in tumor-adjacent tissues and glioma tissues.The activity of miR-146a was up-regulated by the method of lipofection of the mimic of miR-146a.Real-time PCR was also used to observe the expression level of miR-146a.Cell viability analysis was done by MTT.The targeted genes of miR-146a in glioma were scanned by the online targeted gene prediction software.Results: miR-146a was detected down-regulated in glioma tissues,with 35% for tumor-adja-cent tissues(p0.01).The cell viability was decreased after miR-146a was up-regulated in glioma cell by miR-146a-mimic transfection.Notch1 was the potential targeted gene of miR-146a in glioma.Conclusions: miR-146a could affect the proliferation of glioma cell by in-hibiting the Notch1 expression.