2012•PubMedRequires access

[Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro].

Xingjun Yao, Wei Li, Yuehua Wang, Zheng-Bin Ding, Wenzhong Hou, Minmin Zeng, Guangce Deng, Jin-Zhu Zhang, Hongfa Yang

Abstract

OBJECTIVE: To investigate miR-20a expression in human glioma and normal brain tissues and its effect on the proliferation of glioma cells in vitro. METHODS: The expression of miR-20a was detected in human normal brain tissues and glioma tissues by real-time RT-PCR. miR-20a mimics were synthesized and transfected into U251 cells via liposome, and the cell proliferation were detected using MTT assay and flow cytometry. RESULTS: The glioma tissues showed significantly up-regulated expression of miR-20a compared with normal brain tissues (P=0.035). The expression level of miR-20a was higher in high-grade than in low-grade gliomas. miR-20a mimics significantly enhanced the proliferation of U251 cells and the percentage of S-phase cells. CONCLUSION: miR-20a shows potent effect in promoting the growth of glioma cells, suggesting its important role in the pathogenesis of human glioma.

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Key concepts: Glioma, Flow cytometry, In vitro, Transfection, Cell growth, Cancer research, MTT assay, Biology