Effects of miR-181a and miR-181b on proliferation and invasion of glioma cells

Objective To study the effects of miR-181a and miR-181b overexpression on proliferation,transformation.apoptosis,and invasion of glioma cells.Methods The relative expression of miR -181a and miR-181b in human glioma tissue saraples and glioma cell lines were performed by real-time quantification RT-PCR We chemically synthesized miR-181a and miR-181b oligonucleotides and constructed expression vectors for pre-miR-181a and pre-miR-181B.The effects of the proliferation.transformation,invasion,and apoptosis of miR-181a and miR-181b on transfected cells were evaluated via MTT assay,soft agar colony assay,transwell assay and flow cytometric method.Results MiR-181a and miR-181b were strongly down-regulated in all glioma samples and glioma cell lines,and miR-181a expression was negatively correlated with ttunor grades.Overexpression of miR-181a and miR-181b could effectively lead to glioma cells growth suppression,transformation inhibition,apoptosis increase,and invasion reduction.Conclusion The aberrantly down-regulated miR-181a and miR-181b may act as tumor suppressors in human glioma. Key words: MiR-181a; MiR-181b; Proliferation; Invasion; Glioma