New Chemiluminescence Reaction System for Analysis of Polyhydroxy-compounds——Determination of Uric Acid in Urine

A novel flow injection chemiluminescence method for the determination of polyhydroxy -compounds was described. The method is based on that polyhydroxy - compounds enhance the CL reaction of luminol with K7[Cu(IO6)2] in potassium hydroxide medium. In the experiments,luminescent reagent--luminol was injected in order to keep the baseline steady and savereagents,especially,to improve sensitivity. The method was used in measuring uric acid in urineand it allows quantitation of uric acid concentration in the 2×10-8 × 10-6g/mL range with adetection limit of 7.2 × 10-9g/mL. RSD=2.04 % .