Effects of Ginkgo biloba extract on SOD, GSH-Px and MDA in brain tissue of rats during cerebral ischemia and reperfusion

AIM: To observe the effects of Ginkgo biloba extract (GbE)solution on SOD, GSH-Px and MDA in cortex of rats during cerebral ischemia and reperfusion. METHODS: Seventy-eight Sprague-Dawley (SD) rats were divided randomly into thirteen groups: sham control group, intraperitoneal injection of saline; ischemia-reperfusion groups, including ischemia (I) with 2 h/reperfusion(R) 1, 2, 3 h groups, injected intraperitoneally(ip) saline before reperfusion 1 h, respectively; Gb E A treatment groups, including I 2 h/R 1, 2, 3 h groups, ip Gb E A 5 mg·kg -1 before reperfusion 1 h, respectively; GbE B 5,10 mg·kg -1 treatment groups, including I 2 h/R 1,2,3 h groups, ip GbE B 5,10 mg·kg -1 before reperfusion 1 h, respectively. The model of focal cerebral ischemia-reperfusion was established with ligation of rat middle cerebral artery. Xanthine oxidase, DTNB and TBA were utilized for detecting activities of SOD, GSH-Px and contents of MDA in brain tissue, respectively. RESULTS: Comparing determining the ischemia-reperfusion control to treatment groups, GbE B injection can dose-dependently increase the activities of SOD and GSH-Px(P0.05) and decrease contents of MDA(P 0.05). There was no significant difference between GbE B 5 mg·kg -1 groups and GbE A 5 mg·kg -1 groups(P0.05). CONCLUSION: GbE B injection could protect brain from ischemia-reperfusion injury with increase vitality to resist oxidation of brain tissue and decrease content of MDA.