Effect of Vitamin E Combined with Extract of Ginkgo Biloba Leaf on Concentration of Oxidative Related Substances in Peripheral Blood in Rats with Focal Cerebral Ischemia and Reperfusion Injury

Objective To investigate the protective effect and mechanisms of Vitamin E combined with extract of Ginkgo Biloba leaf on focal cerebral ischemia and reperfusion injury in rats. Methods A total of 80 Sprague Dawley rats were randomly divided into control group( n = 40) and observation group( n = 40) to establish models of focal cerebral ischemia and reperfusion injury. The control group was injected with extract of Ginkgo Biloba leaf,while the observation group was injected with extract of Ginkgo Biloba leaf and Vitamin E. A total of 10 rats were selected in each group after ischemia and reperfusion for 1,3,6 and 24 h. The concentration changes of glutathion peroxidase( GSH-PX),superoxide dismutase( SOD),reactive oxygen species( ROS),malondialdehyde( MDA) and platelet activating factor( PAF)in peripheral serum of rats were detected. Results Compared with those in control group,at all time points,the values of GSH-PX and SOD activities were significantly higher,while the values of ROS,MDA and PAF were significantly lower in observation group( P 0. 05). In the two groups,the GSH-PX and SOD activities were decreased when the reperfusion was started,the lowest values were found 6 h after reperfusion,and the increased values were found after reperfusion for 24 h,while the ROS activity and concentrations of MDA and PAF were increased when the reperfusion was beginning,the highest values were found after reperfusion for 6 h,and the decreased values were found 24 h after reperfusion. Conclusion Vitamin E combined with extract of Ginkgo Biloba leaf can relieve focal cerebral ischemia and reperfusion injury by increasing the GSH-PX and SOD activities and reducing the ROS activity and MDA and PAF concentrations in peripheral serum in rats.