2006•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access
Study on the cloning and sequence analysis of bovine FABGL gene
Yun Ma, Shang‐Zhong Xu
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Abstract
The liver tissue from Chinese Simmental cattle was collected to extract RNA and the cDNA of the bovine FABGL gene was determined and analyzed by homology cloning approach combined with RT-PCR,3′ and 5′ RACE in this study.Sequence analysis and bioinformatics study showed that this cDNA contained 994 bp nucleotides,with a 780 bp open reading frame(ORF) flanked by a 16 bp 5′ UTR(uncompletely) and a 198 bp 3′ UTR.The CDS of the obtained bovine FABGL gene showed 89%,89%,87% and 86% identity with the corresponding macaque,human,pig and rat respectively.The deduced amino acid sequence(260 AA) showed 89%,87%,89% and 86% identity with the corresponding macaque,human,pig and rat respectively.Analyzed results for phylogenetic tree of the amino acid sequences of cattle FABGL and other FABGL proteins in human and other animals showed that,among all species mentioned above,the nearest relationship existed between cattle and pig compared to the relationship between cattle and other species.