2008•Journal of Xinxiang UniversityRequires access
Cloning and Analyzing of the Bovine ANGPTL1 Gene in Silicon
Zuo Chun-sheng
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Abstract
The study analyzed the ANGPTL1 sequence of cattle.Sequence analysis and bioinformatics study showed that this cDNA contained 2 576 nucleotides,with a 1 476 bp open reading frame(ORF) flanked by a 135 bp 5'UTR(incompletely) and a 785 bp 3'UTR.The CDS of the obtained bovine ANGPTL1 gene shows 91%,90%,82%,and 93% identity with the corresponding,human,chimpanzee,rattus and dog,respectively.The deduced amino acid sequence(492AA) shows 95%,95%,92% and 95% identity with the corresponding,human,chimpanzee,pig and dog,respectively.Analyzed results for phylogenetic tree of the amino acid sequences of cattle ANGPTL1 and other ANGPTL1 proteins in human and other animals showed that,among all above-mentioned species,the nearest relationship existed between cattle and dog as comparison to the relationship between cattle and other species.