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CLONING AND SEQUENCE ANALYSIS ON IL-2 GENE IN CATTLE

Ran Wang

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Abstract

In order to clone cattle interleukine-2 gene,according to GenBank in the bovine Interleukine-2(IL-2) cDNA sequences,a size of 500 bp cDNA fragment was amplified by RT-PCR from the total RNA extracted by ConA stimulated 12-hours cattle peripheral blood lymphocytes,and then constructed pMD-18T-IL-2 recombinant vector,following identification of PCR and double digestion of EcoR-1 and BamHI.Sequence analysis indicated that cattle IL-2 gene catained an open reading frames(ORF) of 468 bp.Compared the nucleotide with bovine,buffalo,sheep,cat,horse,chicken,canin,human,goat,mice and pig,the homoloaies were 98.9%,98.5%,97.0%,82.4%,79.9%,5.3%,78.2%,79.7%,95.9%,65.2% and 84.7%,respectively.

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What this paper is about

In order to clone cattle interleukine-2 gene,according to GenBank in the bovine Interleukine-2(IL-2) cDNA sequences,a size of 500 bp cDNA fragment was amplified by RT-PCR from the total RNA extracted by ConA stimulated 12-hours cattle peripheral blood lymphocytes,and then constructed pMD-18T-IL-2 recombinant vector,following identification of PCR and double digestion of EcoR-1 and BamHI.Sequence analysis indicated that cattle IL-2 gene catained an open reading frames(ORF) of 468 bp.Compared the nucleotide with bovine,buffalo,sheep,cat,horse,chicken,canin,human,goat,mice and pig,the homoloaies were 98.9%,98.5%,97.0%,82.4%,79.9%,5.3%,78.2%,79.7%,95.9%,65.2% and 84.7%,respectively.

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Available abstract

In order to clone cattle interleukine-2 gene,according to GenBank in the bovine Interleukine-2(IL-2) cDNA sequences,a size of 500 bp cDNA fragment was amplified by RT-PCR from the total RNA extracted by ConA stimulated 12-hours cattle peripheral blood lymphocytes,and then constructed pMD-18T-IL-2 recombinant vector,following identification of PCR and double digestion of EcoR-1 and BamHI.Sequence analysis indicated that cattle IL-2 gene catained an open reading frames(ORF) of 468 bp.Compared the nucleotide with bovine,buffalo,sheep,cat,horse,chicken,canin,human,goat,mice and pig,the homoloaies were 98.9%,98.5%,97.0%,82.4%,79.9%,5.3%,78.2%,79.7%,95.9%,65.2% and 84.7%,respectively.

Key concepts: GenBank, Biology, Complementary DNA, Cloning (programming), Open reading frame, Molecular biology, Recombinant DNA, Gene

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