Changes of cellular immune function with Chaihulongmu decoction in Lewis mice with lung cancer

Objective To study effects of Chaihulongmu decoction on cellular immune function such as T lymphocyte subgroup,NK cell activeness,IL-10 and γ-interferon of Lewis mice with lung cancer. MethodsThe cells of Lewis lung cancer were planted in the right axilla of C57BL/6J inbred strain mice subcutaneously.The mice with cancer were randomly divided into model group(MG),herbal group(HG),DDP group(DG) and combination group(CG).The tumors were weighed when the mice were sacrificed in each group.Then the inhibition rate of tumor was calculated.The experiment of the lactic dehydrogenase(LDH) releasing was used to detect the activity of NK cell in the mouse spleens.The method of enzyme-linked immuno sorbent assay(ELISA) was used to detect the IL-10,γ-interferon in the supernatant fluid of spleen.The rates of CD4,CD8 and the ratio of CD4/CD8 were detected by the flow cytometry(FCM) in the way of PE/FITC fluorescent staining in the spleens of mice. ResultsThe inhibited effectiveness of CG was optimal,and the inhibition rate of tumor was 62.4%.There was significant difference comparing to DG and HG(P0.01).The activity of NK cell in HG was highest,and the difference was obvious comparing to MG,DG and CG(P0.01).The content of IFN-γ was 65.78±17.68 in HG,which was higher than that in DG(P0.05).The content of IL-10 in MG was highest(153.30±33.14).In HG it was lower than MG,DG and CG(P0.05).The figure of CD4+ and the ratio of CD4+/ CD8+ in HG were the highest.Comparing to the MG and the DG,the difference was extremely obvious.(P0.01).While the percentage of CD8+ cell in each post-treatment group had no obvious changes. ConclusionThe prescription of Chaihulongmu decoction can inhibit tumor and improve the cellular immune function through raising the level of IFN-γ,reducing the superior expression of IL-10,strengthening the activity of the NK cell,obviously elevating the percentage of the CD4+cell and the ratio of CD4+/CD8+.