Observation of anti-tumer effect of Trichinella spirialis in mice on A549 lung cancer cell

Objective To observe the anti-tumer effect of Trichinella spirialis on A549 lung cancer cell.Methods The BALB/c mice were divided into 7 groups at random,in which there are 10 mice in each group.Group 1-6 of mice were infected with T.spirialis that attenuated by different methods and inoculated with A549 lung cancer cells in different times;Dissected the mice after 30 d,the anti-tumer effects of T.spirialis was observed by determining the size and weight of tumer,the number of CD3+,CD4+,CD8+ and CD4+/CD8+ of the mice.Results The size and weight of the tumor in the mice which were infected by T.spirilas、violet attenuated T.spirilas and Co60 attenuated T.spirilas were smaller than the control group(P0.01)).The percentage of CD3+,CD4+,CD8+ in spleen and CD4+/CD8+ and no tumor occurance ratio in three test groups were higher than the control group(P0.05;P0.01).The anti-tumer effect of the T.spirialis infected mice in different times was obviously different,the size and weight of the group inoculated the A549 lung cancer cell before the 7th day of T.spirialis infected and after 11 d of the infection with T.spirialis were smaller and lighter than the control group(P0.01);The percentage of CD3+,CD4+,CD8+ in spleen and CD4+/CD8+ were higher than the control group(P0.05).Conclusion The results showed that T.spirialis attenuated by different methods and the non-attenuated T.spirialis inhibit the growth of A549 lung cance in vivo;anti-tumer effect in which the A549 lung cancer cell inoculated after the 11 days of T.spirialis infection was better than the ahead of 7 d.