Study on the optimization of the extraction conditions and the anticancer activity of polysaccharide from the highly-yielding strain Cordyceps Militaris N102

The polysaccharide in highly-yielding mutagenic strain Cordyceps militaris N102 was studied.Response surface methodology(RSM) was applied to optimize the extraction conditions of polysaccharide in Cordyceps militaris N102 mycelium.After repeated freeze-thaw,deproteinization,dialysis and ethanol precipitation,crude polysaccharide of Cordyceps militaris N102(2FPS,4FPS and 6FPS) was obtained.The MTT assay was applied to study the inhibitory activity of crude polysaccharide of Cordyceps militaris N102 on the Hela cells proliferation.The results indicated that the optimum process conditions for the extraction conditions of adenosine were as follow:extracting temperature was 83℃,extracting time was 3.2h and solvent/solid ratio was 52:1(mL:g).The average yield of adenosine in 3 validation experiments was 7.24%.The relative error was 1.16%.The results of MTT assay showed that polysaccharides presented inhibitory activity on the cells proliferation of Hela.After 72h incubation,the inhibition rate of Hela was 74.98%,and the IC50 was only 1.72mg/mL.