Effects of Bushen Huoxue decoction on in-vitro nuclear maturation of immature PCO oocytes in mice

Objective To investigate the effect of Bushen Huoxue decoction on in-vitro nuclear maturation of immature PCO oocytes in mice.Methods ⑴ The serum containing Bushen Huoxue decoction was prepared.⑵The mice models with polycystic ovary were made.⑶The immature oocytes isolated from the polycystic ovaries of the mice in the germinal vesicle(GV) were cultured with the serum containing decoction collected from different time respectively and the relation of GVBD and PB1 extrusion were detected.Results ⑴GVBD after 2h and 2.5h were higher in the serum containing decoction group than that in the controls and normal serum groups,and the difference was the most notable after 4th hours(P0.01).⑵ The PB1 in 2 h and 2.5 h in the serum groups were higher than that in the controls and normal group,and the difference was the most notable after 18th hours.Conclusion The serum containing Bushen Huoxue decoction after 2～2.5 h is very effective to promote in-vitro nuclear maturation of immature oocytes significantly in PCO mice.